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Technology Overview

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Illumina Sequencing is the most widely used Massively Parallel Sequencing (MPS) / Next-Generation Sequencing (NGS) technology worldwide. It is a high-throughput approach to DNA sequencing which based on sequencing by synthesis (SBS) chemistry. It involves the use of bridge PCR to clonally amplify the library on the flow-cell and supports single read or paired-end read. Indexed adaptors allow multiplexed sequencing on a single lane of a flow cell for time- and cost- effective sequencing run. This technology enables a wide variety of applications for genomics, transcriptomics and epigenetics studies.

Our Core has recently acquired the NovaSeq 6000, the most advance High throughput Sequencer from Illumina. The NovaSeq 6000 system offers unprecedented throughput at the lowest per Gb data cost, it is compatible with existing library preparation kits for other Illumina Sequencers, supporting a wide range of sequencing applications.

Our Core also hosts the MiSeq sequencer, which supports small scale projects that need rapid data delivery or longer sequencing read length. With the latest hardware and software configurations, MiSeq supports a variety of read lengths, from SE36 (1 x 36 bp) to PE300 (2 x 300 bp). Please contact us for details.

The Core offers full service for Illumina sequencing, including library preparation and sequencing run.  Sequencing run service for pre-made library is also available.  Please enquire.

 

Application:

Below table summarized the services done in the Core since Apr-2012.

Sample Type Service Type
DNA   Whole Genome Sequencing
DNA   Human Exome Sequencing
DNA   Targeted Enrichment Sequencing
DNA   RainDance Amplicon Sequencing
DNA   ChIP Sequencing
DNA   Shotgun Metagenomic Sequencing
DNA   16S Metagenomic Sequencing
DNA   Reduced Representation Bisulfite Sequencing (RRBS)
DNA   Whole Genome Bisulfite Sequencing (WGBS)
RNA   PolyA+ mRNA Sequencing
RNA   rRNA depleted RNA Sequencing
RNA   Small RNA Sequencing
RNA   PAR-CLIP Sequencing

  Illumina NovaSeq



  Illumina MiSeq

 

Contact

  Mr Wilson Chong
  Mr Carlos Choi
  Mr Clement Chow
  Mr Johnny Lam
  Mr Chengwei Wu
  Mr Wilson Chan
  Ms Tiky Leung

  Telephone: 2831 5486 / 2831 5477 / 2831 5467 / 2831 5479
  Email: nextgenseq.cpos@hku.hk