iScan BeadArray Technology Overview

Illumina iScan System utilizes BeadArray technology for a broad range of DNA analysis applications including genotyping and methylation studies. The 3-micron silica beads are assembled in microwells on slide with high density, enabling multi-sample per slide. Each bead is covered with hundreds of thousands of copies of a specific oligonucleotide that act as the capture sequences in one of Illumina’s assays. Different combinations of probe design enable a wide range of applications on a whole-genome scale, including gene- and exon-level analysis, genotyping and resequencing.

The iScan platform at the Core can perform the following analysis:

• Whole genome SNP genotyping
• Whole genome methylation analysis
• Whole genome copy number variation (CNV) analysis
• Custom SNP genotyping

A typical iScan BeadChip experiment (Infinium) includes the following steps:

• Preparation of DNA isolated from specimens
• Hybridization of sample to BeadChips
• Stringent washes, single base extension, staining and imaging of BeadChips
• Analysis of scanned images and data extraction

For details of iScan BeadArrayTechnology and products, please visit Illumina iScan website.